manufacturer :       Waters

model :                   2487

annotation :           Dokumente engl.

Waters 2487 detector is the most sensitive and versatile absorbance detector available for HPLC. With easy programming from the front panel keypad, timesaving qualification derived from the built-in cuvette capability, and dual wavelength operation, the 2487 detector will enhance the productivity of any chromatography laboratory. The 2487 detector can operate either as a stand-alone, modular UV/Vis absorbance detector or an integral component of several Waters HPLC Systems. All performance specifications are for the 2487 detector following a warm-up period of one hour.

Innovative capabilities

·                      The patented* TaperSlit- flow cell maximizes energy throughput, eliminates refractive index effects, and provides consistent resolution over the entire wavelength range, ensuring the highest sensitivity while maintaining optimal spectral performance.

•            Lamp Optimization Software in the 2487 detector automatically maximizes signal-to-noise ratio in visible and UV wavelength ranges using a single lamp. This feature extends the lifetime of the lamp for consistent results over time. Because only one lamp needs to be maintained, the 2487 detector requires less maintenance and is more cost effective. Optimization is performed automatically on power up or with push-button re-calibration and requires no manual adjustments to the detector.

•            Dual wavelength mode simultaneously monitors absorbance at two user-selectable wavelengths, giving you additional analyte information and increasing your productivity.

System operation or stand-alone

Two pairs of analog outputs can be independently programmed (e.g. one for absorbance and one to monitor sample energy or your chromatogram at a different sensitivity or filter setting). The 2487 detector can be controlled by Waters data products when included in Waters HPLC Systems.

As a stand-alone module, the 2487 detector lets you construct time-based programs with full control of major parameters. You can program and store up to ten methods (16 steps each) and five spectra. It also has the capability to operate in dual wavelength mode.

Simple performance maintenance

With the 2487 detector, you can count on maximum performance with minimum maintenance.

•            All mirrors are accessible by using a screwdriver so there’s no need to open the optics bench

•            The flow cells are interchangeable using only three screws

•            The lamp is pre-aligned and requires the use of only two screws

•            Lamp serial numbers and number of ignitions are automatically tracked

•            On-board signal-to-noise tester

Exceptional versatility for HPLC applications

The 2487 detector provides optimal performance for analytical, preparative, and microbore applications, whether you work in R&D, Analytical Development, or QA/QC.

Analytical

•            Wavelength verification and calibration via an internal erbium filter (patent pending for the use of an erbium filter in wavelength calibration of HPLC detectors) enables calibration to within ±1 nm utilizing four wavelength maxima, which covers the UV as well as visible wavelength range.

•            MaxPlot speeds method development by maximizing response from two wavelengths and integrating these wavelengths into one chromatogram, even when retention times are unknown and spectra are dissimilar.

•An integrated cuvette holder, coupled with Waters qualification workbooks and optional cuvette kits for accuracy and linearity, make qualification easier and faster. Ideal for methods development, the cuvette capability helps you quickly obtain your analyte absorbance spectrum.

Preparative

•            Advanced fraction collection can be activated automatically, based upon time or absorbance
thresholds, and is made easier with real-time views of chromatograms and spectral scans.

•            RatioPlot divides absorbances at two selected wavelengths and plots the resulting ratiogram to help detect hidden components that may be present under individual peaks. It can also trigger threshold events.

•            Log absorbance and difference can be plotted enabling both small and large peaks to be viewed on a single chromatographic output.

Microbore

•            No volume-adding bulkhead fittings mean minimized band broadening, which maintains excellent peak resolution. This convenience, combined with the low cell volume of our optional, microbore TaperSlit flow cell means optimal analyses for narrowbore and microbore applications.

•            New optical design increases light throughput, resulting in an overall decrease in baseline noise for better sensitivity in microbore applications. In addition, the single wavelength mode is optimized for high sensitivity and offers absorbance measurements with the highest level of performance and ease of use available today.

Operating Specifications

Wavelength Range                                    190-700 nm

Light Source                                               Deuterium arc lamp

Bandwidth                                                  5 nm

Wavelength Accuracy                                ±1 nm

Wavelength Repeatability                          ±0.1 nm

*Linearity                                                    ≤5% at 2.5 AU propylparaben, 257 nm

*Baseline Noise, Single Wavelength         <± 0.35 x 10 ^-5 AU, dry cell, 254 nm, 1 sec. TC

*Baseline Noise, Dual Wavelength            <± 2.5 x 10 ^-5 AU, dry cell, 254, 280 nm, 2 sec. TC

Drift                                                            1 x 10 ^-4 AU/hour

Measurement Range                                 0.0001 - 4.0000 AUFS

Filter Setting, Single Wavelength Mode     0.1-5 seconds (Hamming)

                                                                  1-99 seconds (optional RC)

Filter Setting, Dual Wavelength Mode       1-50 seconds (Hamming)

                                                                  1-99 seconds (optional RC)

Flow Cell Design                                        TaperSlitTM (patented**)

Path Length                                                10 mm (standard analytical cell)

Cell Volume                                                10 µL (standard analytical cell)

Pressure Limit                                           1000 psi (standard analytical cell)

Wetted Materials                                        316 stainless steel, fused silica, Teflon®

Optional TaperSlit Flow Cells                    Semi-preparative, Inert (Titanium)

                                                                  High pressure for LC/MS, Microbore

Memory                                                      10 time-programmed methods, 5 complete spectra

Analog Outputs                                          Two, software selectable: Absorbance Energy, Log

                                                                  Absorbance, RatioPlot, MaxPlot

Inputs                                                         Remote Chart Mark, Auto Zero, Lamp On/Off,

                                                                  and Inject Start

IEEE-488 Bus                                            Standard for use with Waters Data Products, System Controllers, and Instruments

Power-up Diagnostics                               Optics and electronic diagnostic routine

Lamp On/Off Timer                                   Front panel, remote event input, or time programmable

Power-up Wavelength Verification            Automatic on power up via internal erbium

                                                                  filter (patent pending) and D ₂ lamp

4-Point Wavelength Calibration                 On demand via internal erbium filter and D2 lamp

Physical/Environmental Specifications

Dimensions                                                Width: 1 1 .2 inches (28.4 cm)

                                                                  Height: 8.2 inches (20.8 cm)

                                                                  Depth: 19.8 inches (50.3 cm)

                                                                  Weight: 20.5 pounds (9.3 kg)

Operating Temperature                             +4° C to 40° C

Operating Humidity                                    20 to 80%, non-condensing

Audible Noise                                             <70 dB (A)

Electrical Specifications/Needs

Power Requirements                                 100 VAC (85-110 V)

120 VAC (102-132 V )
220 VAC (187-242 V)
240 VAC (204-264 V)

Line Frequency                                          50 Hz (47 to 53 Hz)

                                                                  60 Hz (57 to 63 Hz)

Power Consumption                                  145 VA (nominal)