This offering includes the Thermo Scientific Vanquish UHPLC System. The instrument is in perfect working condition and ready to use. Overview: Thermo Scientific Vanquish UHPLC Systems. 1. Autosampler. Purpose: Performs automatic sample introduction into the mobile phase stream. Acquires samples from vials, plates, or tubes and injects them with high precision (often in the nL to µL volume range). 2. Pump Module. Purpose: Delivers the mobile phase (solvent) through the system at high pressure. Binary Pump: Mixes two solvents under high pressure, ideal for fast gradients. Quaternary Pump: Mixes up to four solvents at low pressure, offering more flexibility but slower gradients. Controls the flow rate (e.g., 0.001–5,000 mL/min) and enables gradient operation (changing solvent composition over time). 3. Detector Module (x2). Purpose: Detects and measures analytes as they leave the column. Available detectors: - Diode array detector (DAD/UV-Vis): most common, detects UV-active compounds. - Fluorescence detector: for fluorescent compounds. - Charged aerosol detector (CAD): universal detection for non-UV active compounds. - Refractive index detector: for sugars and polymers. - Mass spectrometer (MS): advanced, highly sensitive detection. The Thermo Fisher Vanquish system offers different versions for different analytical needs – from routine HPLC to ultra-high-throughput and low-flow LC–MS applications: - Vanquish Flex UHPLC: Flexible method development for HPLC/UHPLC, up to 1000 bar operating pressure, supports binary, quaternary, and dual pumps, including intelligent features such as solvent monitoring and an optional touchscreen. - Vanquish Horizon UHPLC: Maximum performance with up to 1500 bar, minimal gradient delay volume, biocompatible flow paths, and excellent retention time stability and detector sensitivity. - Vanquish Neo UHPLC: Designed for modern low-flow LC-MS applications (nanoflow to microflow), offering high sensitivity and robustness. - Vanquish Analytical Purification LC: Specifically designed for precise fractionation, with a high pressure range (700–1500 bar), adjustable flow up to 10 mL/min, integrated fraction collector, multimodal detection, and advanced automation. Key platform features: - Modular design: Selection of components such as pumps, samplers, detectors, and temperature control units for customized system configuration. Most models feature biocompatible flow paths and Viper finger-tight connections for low dead volume and tool-free assembly. - Temperature control: High-performance column compartments with active/passive heating, preheating, and post-column cooling to control frictional heat and improve reproducibility. - Detection options: Available detectors include diode arrays with LightPipe optics for excellent sensitivity, CAD for virtually universal detection, fluorescence, multi-wavelength, refractive index, and mass spectrometry detection. - Software integration: Fully compatible with Chromeleon CDS (including SII for simplified MS integration) as well as with Xcalibur and TraceFinder for LC-MS workflows. - MS integration: Seamless interfaces to Thermo MS platforms – triple quadrupole and Orbitrap – for sensitive and precise mass analysis. - Economic efficiency: Thermo emphasizes reduced unplanned repairs, increased productivity, and climate ambitions with renewable energy in manufacturing.