The Thermo Scientific PikoReal 24 Cycler is ideal for getting started with real-time PCR with small sample numbers (24-well format). The PikoReal software enables simple and intuitive operation of the cycler, as well as convenient online control via a computer. The cycler features five detection channels that detect hydrolysis probes (5'Nuclease/TaqMan) as both single-color and multiplexed samples. Thermal block unit: - Block format: 24 wells. - Sample volume: 10–50 µl. - Consumables: Low-profile strip tubes and PCR tubes. - Maximum heating rate: > 5 °C/sec. - Maximum cooling rate: 4.5 °C/sec. - Temperature range: 4–99.9 °C. - Temperature accuracy: ± 0.2 °C. - Temperature uniformity: ± 0.3 °C at 95 °C, ± 0.15 °C at 60 °C, ± 0.2 °C at 72 °C. Heated lid:. - Temperature range: 50 – 110 °C. - Control: Automatic temperature and pressure adjustment. Optics:. - Excitation: 5 LEDs. - Excitation range: 475 – 640 nm. - Detection: CCD. - Detection range: 520 – 740 nm. - Measurement time: all channels < 13 s, individual channel < 3 s. - Filter wavelength ranges:. Channel 1 – Excitation: 475 – 500 nm, Emission: 520 – 550 nm. Channel 2 – Excitation: 515 – 535 nm, Emission: 557 – 590 nm. Channel 3 – Excitation: 570 – 590 nm, Emission: 615 – 650 nm. Channel 4 – Excitation: 600 – 640 nm, Emission: 666 – 740 nm. Channel 5 – Excitation: 475 – 500 nm, Emission: 520 – 590 nm. - Multiplex: up to 4 targets. - Dynamic range: 10 log steps. - Sensitivity: 1 copy. Analysis modes: - Absolute quantification. - Relative quantification. - Melting curve analysis. - Allele discrimination. - High Resolution Melt (HRM). Standalone or PC-controlled operation:. The PikoReal qPCR system is controlled by the PikoReal software, which enables remote monitoring of qPCR experiments online from your own computer. Alternatively, the qPCR protocol can be saved to a USB stick and loaded directly into the PikoReal device in standalone mode. The software includes modules for absolute and relative quantification, melting curve analysis, allele discrimination, and high-resolution melting (HRM). Advanced algorithms ensure easy data normalization without the need for external reference dyes. Dimensions (W x D x H): 300 x 230 x 310 mm. Weight: 9.2 kg. Software included.