Description. Symbiosis Pro:. This is Spark Holland's unique solution for integrated online SPE-LC-MS automation (XLC-MS). Enables high flexibility in processing different sample types by selecting one of three fully automated operating modes: LC-MS (direct LC without SPE), XLC-MS (online SPE coupled to LC-MS) and AMD (advanced method development). Specifically designed for high-throughput pharmaceutical analysis, this system combination of highly selective online SPE and a first-class top-line autosampler is the perfect tool for direct injection of raw biological samples. The lossless sample injection mode delivers optimal results even when only a small sample volume is available (5 µL injection from an 8 µL sample). Symbiosis online SPE systems process multiple batches/test series quickly and reliably, with the highest analytical performance, and fully automatically. You also have all the tools for method development using a systematic approach that produces a working online SPE method within days. These versatile workhorses are a safe investment and allow you to run more projects with the highest assay performance in the same amount of time while leveraging full MS capacity for the economic benefit of the lab. Reliable injection performance. Well plates and vials. Sample cooling. HPLC and UHPLC version. Stackable. Simultaneous sample preparation & LC. Automatic solvent mixing. Multiple assay processing. Integrated software control in SparkLink, Analyst, Xcalibur and Masslynx. More samples per day per analyst. Generic templates for acidic, basic and neutral compounds. Instant feedback during method optimization. Test more variables in less time. No more compromises on assay quality. Mistral:. Almost every parameter that defines a chromatographic separation is temperature dependent. Therefore, temperature control provides an indispensable tool for optimizing your HPLC or UHPLC assay. It can be seen that at constant temperature retention times are more consistent, but varying the temperature can also be a great help to increase selectivity, improve peak shape and reduce analysis time. High temperatures significantly reduce column back pressure, allowing higher solvent flow rates, narrower columns or smaller particles. However, such benefits will only occur if the temperature itself is stable and precise and there are no temperature gradients in the oven or column. This is exactly what we had in mind when we designed the MISTRAL with its forced air oven, excellent temperature control and integrated solvent preheater. 5 – 90°C. Temperature gradient programming. Excellent temperature stability. Integrated solvent preheater. Vapor sensor. Automatic column selection. High pressure doser:. Traditional volumetric dispensers are designed to fill reagents or samples into vials or wells at ambient pressure or a few bars at most. However, online dispensing of volumetric reagents may require the ability to introduce reagents into a system with high working pressure. The HPD was originally developed by Spark for SPE solvent delivery in online SPE-LC systems, but other applications are also conceivable. Think of pre-column reagent delivery for online derivatization in HPLC, post-column reagent delivery for detection amplification, etc. Accurate volumetric dispensing at controlled flow rates. Independent of back pressure up to 300 bar. Choice of 4-24 solvents or samples. Online solvent mixing. ACE:. Combines the best of the online and offline SPE worlds. Offline SPE (Solid Phase Extraction) allows cartridge renewal for each sample, avoiding the risk of retention changes and carryover. On the other hand, the low pressure resistance limits the use of small sorbent particles. Offline elution of the sample requires collection and rearrangement (often including evaporation and reconstitution) into autosampler vials or wells. And finally. Injection into the LC system. Furthermore, at least one manual transfer of collected samples from the SPE robot to the LC system is required. Online SPE (or column switching, or 2D LC) allows the use of small sorbent particles for efficient SPE as well as elution of the sample directly into the LC system through the mobile phase. Maximum SPE efficiency as well as maximum SPE-LC automation. But. Column switching does not allow frequent automated renewal of the SPE column or cartridge. The Spark ACE eliminates this disadvantage. It selects a fresh cartridge as often as desired and seals it against the pressure of your LC system for online elution. ACE combines the flexibility of offline SPE with the power and automation of online SPE. Disposable SPE cartridges sealed up to 300 bar. Single-use or reusable SPE cartridges - your choice. Wide range of SPE chemistries. Two trays of 96 cartridges each as standard. The system consists of: Spark Conditioned Stacker Model 931Spark Autosampler Model 930Spark LC-Pump Model 750 (x2)Spark High Pressure Dispensor Model 730 (x2)Spark ACE Model 725Spark Mistral Model 885