The LS50B is a fluorescence/luminescence spectrophotometer with a high-pressure pulsed xenon source. It is equipped with monochromators on both the excitation and emission sides and can perform both excitation (200-800nm) and emission (200-900nm) scans. The Perkin Elmer software offers four modes of operation: single scan, accumulation (for use with weak fluorophores or low concentration), kinetic, or 3D mode. The sample holder has no temperature control. The software runs under Windows XP and the workstation is part of the laboratory network. The printout is done via the laboratory's workgroup printer. Electronic data can be accessed either via a USB stick or via email as an attachment. Specifications:. Principle: Computer-controlled ratio luminescence spectrometer capable of measuring fluorescence, phosphorescence, chemiluminescence and bioluminescence. Source: Xenon discharge lamp, equivalent to 20 kW for 8 µs duration. Pulse width at half height <10 µs. Sample detector: Pulsed photomultiplier with modified S5 response for operation up to approximately 650 nm. An optionally available red-sensitive R928 photomultiplier can be installed for operation up to 900 nm. Reference detector: Pulsed photomultiplier with modified S5 response for operation up to approximately 650 nm. Monochromators: Monk-Gillieson type monochromators cover the following ranges:. Excitation 200-800 nm with selectable zero order. Emission 200-650 nm with standard selectable zero-order photomultiplier, 200-900 nm with optional R928 photomultiplier. Synchronous scanning is available, with either constant wavelength or constant energy difference. Wavelength accuracy: +1.0 nm. Wavelength reproducibility: +0.5 nm. Spectral bandwidth: The column for excitation (2.5-15.0 nm) and emission (2.5-20.0 nm) can be varied and in 0 .1 nm steps can be selected. Phosphorescence mode: Delay and gate times can be varied to measure phosphorescence. Minimum total period 13.0 ms (50 Hz). Scan speed: Scan speed can be selected in 1 nm increments for 10-1500 nm/min. Time-dependent data can also be collected. Emission Filter: Computer selectable cut-off (high pass) filters at 290, 350, 390, 430 and 515 nm, a blind filter (as a shutter), a 1% T attenuator and a clear beam position. Sensitivity: Signal-to-noise ratio is 500:1 RMS, using the Raman band of water with excitation at 350 nm, excitation and emission bandwidth 10 nm. Standard cuvette holder: A single position thermostat for 10 mm Labprocure cuvettes carries the Responsibility for this device.