More Information
Objektnummer	B00014618
ID-number	014618
Object name	Nuclisens PCR
Status	Archiv

Status, terms of delivery and payment

Verification of devices

The second-hand devices are verified by Labexchange Service GmbH before delivery. You are receiving only fully functional devices.

Dispatch time

The stated dispatch times are the shortest possible ones for each article. The effective dispatch times can vary. The effective dispatch times will be stated in the order confirmation.

As a matter of principle, we are offering collective deliveries. The shipping time is calculated based on the position with the longest lead time. A partial delivery is possible on explicit request.

Shipping methods

Parcel services, forwarding agencies, self-pickup, delivery by Labexchange fleet.

Delivery information

Prices exclude shipping costs. Stated shipping costs are to be expected. Deviations are possible. If transport costs are not specified, please ask separately for them.

The stated transport and packing charges apply to the most favorable route if transport and are to be understood as subject to verification due to unexpected cost increases. By reason of unpredictable events, cargo rates and delivery times can change at any time and therefore have to be adapted to the recent situation. Import formalities and possible customs charges will be borne by the purchaser. Incoterm coding according to Incoterms 2010: For persons who collect the devices themselves: EXW, for dipatch by sea: CFR, by air freight: CPT, other shipments: DAP. Note for international shipments: A proof of preference/EUR1 will not be issued by us. When self-collecting/ordering EXW from countries within or outside the European Union, 16% VAT will be retained as a deposit until we have received the corresponding confirmation of arrival/bill of delivery from the buyer.

Terms of payment

We do not accept payment by letter of credit, PayPal, etc. In each case the invoice amount is payable without deduction. Discount is not granted.

Country	Possible payment methods	Comment
DE, AT, CH	Payment by invoice, payment in advance, paymet by credit card	Payment by invoice is only possible for corporate clients.
NL, BE, LU	Payment by invoice, payment in advance, payment by credit card	Payment by invoice is only possible for corporate clients
Other countries	Payment in advance, payment by credit card

Our General Terms of Sale, Delivery and Payment are valid and are available for download here.

The goods are offered subject to prior sale.

Definition of status

All articles are used articles, except an article is listed especially as a new device.

Status	Condition	Comment
Immediately available	Used	The article is fully functional and in impeccable condition. It can be shipped immediately.
In stock	Used	The article is on stock. Our service technicians will verify the article before delivery. You are receiving only a fully functional article.
Published	Used	The article is still with the provider. After your order the article will be purchased and verified by us before being shipped to you. A certificate of operativeness as well as a service report are included in delivery.
New device	new	The article is brand new and unused. Regarding new equipment the guarantee/warranty conditions of the corresponding manufacturer apply.
Labprocure	Used	Labprocure GmbH, as the advertiser, is responsible for the content of this device offer. Labprocure assumes liability for the offers advertised here and for the photos and offer texts included. Labprocure GmbH, Bruckstraße 58, 72393 Burladingen.

Firma: Nuclisens

Modell: PCR

Kommentar: Dokumente engl.

The following illustrations and descriptions are referring to the instrument model and are drawn from brochures. They are not representing the delivery volume. The exact delivery content you will find only in the offering text.

Introduction

The NucliSens System consists of the Nucleic Acid Sequenced Base Amplification (NASBA) technology combined with a simple nucleic acid extraction procedure, NucliSens Extractor, and a user-friendly detection method, NucliSens Reader. The NucliSens Extractor automates the Isolation process for the nucleic acids. This instrument relieves the user of the mang centrifugation, Suspension, and aspiration steps. lt allows the processing of multiple samples during a single Run, minimizes the labor and reduces the time required for the processing steps.

Intended use

For in vitro diagnostic use.

The NucliSens Extractor is an instrument for the extraction of nucleic acids from lysed biological samples; the nucleic acids are concentrated by adsorbing to Silica, filtering, washing, and eluting into a closed vial for further processing.

The extraction and concentration of the nucleic acids are performed in a 'closed" Container, referred to as the nucleic acid extractor Cartridge. The Cartridge is designed to hold the lysed biological sample, Silica, and internal control nucleic acid (if used), during the extraction process. A ready source of filtered, dry air provided to the Cartridge allows fluid manipulation for the filtration, washing and elution steps, and for drying of the Silica following the wash steps. This minimizes contamination of the nucleic acids and exposure of the operator to hazardous biological samples.

General Theory of Operation

The technology employed in the NucliSens Extractor is based on the manual method by R. Boom et al. (1990, J. Clin. Micro. 28, 495-503), which has been successfully used to isolate nucleic acids from a variety of biological samples. The 'Boom" method utilizes the lysing properties of a chaotropic agent and the nucleic acid binding properties of a solid phase, Silica, in the presence of this agent.

The extraction procedure is initiated by adding the biological sample to Lysis Buffer. The Lysis Buffer disrupts (dissociates) the cells and/or viral particles, releasing the nucleic acids and inactivating the nucleases present in the mixture. Silica is added to the lysed mixture for adsorption of the nucleic acids prior to processing by the Extractor. (When calibrator or control nucleic acids are used, these materials are added prior to the addition of Silica.) This mixture, containing sample (nucleic acids), Lysis Buffer, and Silica, is added to the Cartridge which is subsequently placed on the Extractor for processing. The Silica (solid phase) containing the adsorbed nucleic acids is filtered from the mixture, washed to remove extraneous materials, and dried. The adsorbed nucleic acids are eluted from the Silica into a closed Eluate Cup for further processing.

Extractor applications

A pure beginning for endless possibilities

The NucliSens Extractor can be used to prepare nucleic acids from many sample types for a wide range of applications. The unique features of the Extractor make it extremely well suited for applications in the fields of clinical, in vitro diagnostic testing and blood safety testing. The Extractor is the instrument of choice as front-end for:

HucliSens HIV-1 OT

The NASBA based assay for HIV-1 RNA quantification. Automated extraction ensures a high degree of standardization and labor-efficiency.

HIV-1 RIIA Genotyping

One eluate from the Extractor/HIV-1 QT assay can not only be used for HIV-1 RNA quantification but also very effectively for HIV-1 RNA sequencing. to determinate antiviral drug resistancy.

The Extractor allows excellent sensitivity of genotyping in clinical samples with low RNA concenfrations.

HucliSens HIV-1 OL

The quantitative NASBA assay for detection of HIV-1 RNA.

HucliSens CMV pp67

The NASBA-based assay for CMV mRNA detection.

HucliSens Basic Kit

The standardized platform for customized RNA amplification testing. Please ask for available applications.

Plasma Pool testing (HAT)

The Extractor features very effective isolation of vital targets for plasma pools in blood safety testing. Extraction from 2 ml of the pool allows very sensitive down-stream testing for HCV-RNA, HIV-1 RNA, HBV-DNA, and Parvo B19-DNA. As of July 1999, the Extractor has been approved by the German Paul-Ehrlich Institute for minipool screening of blood donations for HCV-RNA, for a maximum: ppol size of 96 donations. (6)

The superior sensitivity of the Extractor in combination with pool testing results in costeffective nucleic acid testino: the costs per blood donation are strongly reduced as compared to single donation testing.

Technical Specifications

Physical

* Height: 79 cm

* Diameter 66 cm

* Weight 90 kg:

Electrical

* Voltage: 100, 120, 220, 240 V ± 10%

* Current 7.0, 5.0, 2.5, 2.5 A

* Frequency 50 - 60 Hz

* Power 700W Maximum

* Fuses: 10 A Time Detay (100/120 V)

6,3 A Time Delay (220/240 V)

Environmental conditions

* Operating temperature 15-30 °C

* Storage temperature 10-50 °C

* Humidity 80% maximum

Noise generated

* Typical Less than 55 dB(A)

* Maximum: Less than 61 dB(A) (duration < 10%)

EMC Compliance

* CE 89/336/EEC, Class A (essential req.)

* Emissions: EN 50081- 1 : 1992 (class radiated emissions)

* Immunity: EN 50082-1 : 1997

Electrical safety complicance

* International Standards:>

UL 3101 -1 (Harmonized UL 1262-1 and IEC 1010-1),

CSA C22.2 No. 1010.1-92

CB Certified and Construction File

Product group: PCR

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