Description. The Ion Personal Genome Machine (PGM) System combines semiconductor sequencing technology with natural biochemistry to convert chemical information directly into digital data, making sequencing accessible to your lab. This established system leverages the exponential advances in the semiconductor industry (known as Moore's Law) to provide scalability and flexibility for diverse applications. The Ion PGM System uses natural sequencing chemistry, eliminating the need for expensive optics and reducing complex chemistries to measure natural DNA extension. The system uses direct, real-time sequencing detection and typically delivers sequencing results in 3 to 7 hours. Processor: Dual 6-core 2.66 GHz processors. Capacity: 1 chip per run. Chip compatibility: Ion 314 Chip v2 BC, Ion 316 Chip v2 BC, Ion 318 Chip v2 BC. Current limit: 6.7 A (max). Data output: BAM files, FASTQ files, SFF files, VCF files. Display interface: Touchscreen user interface. External computing: Torrent server. High-throughput compatibility: Compatible for high-throughput, multiplexing. Memory: 48 GB RAM, eight 2 TB hard drives in RAID 5. Operating system: Ubuntu 10. 04. Output: Ion 314 Chip v2: Up to 100 Mb, Ion 316 Chip v2: Up to 1 Gb, Ion 318 Chip v2: Up to 2 Gb. Read length: 35-400 bases (average: 200 bases). Read/run: Ion 314 Chip v2: 400-500 thousand, Ion 316 Chip v2: 2-3 million, Ion 318 Chip v2: 4-5.5 million. Run time: Ion 314 Chip v2: 2.3 to 3.7 hours, Ion 316 Chip v2: 3.0 to 4.9 hours, Ion 318 Chip v2: 4.4 to 7.3 hours. Sequencing scale: 3 Mb to 2 Gb. Technology: Afterlight Ion Semiconductor Sequencing. Throughput: Up to 2 Gb per run. Voltage limitation: 100 V (min) to 240 V (max). Dimensions (H x W x D): 530 x 240 x 200 mm. Weight: 30 kg. The Ion OneTouch 2 system performs template amplification and enrichment as part of the manual workflow for the Ion PGM, Ion Proton, and Ion S5 and Ion S5 XL systems. It offers scalable template preparation for all Ion Semiconductor chips. With its small footprint, the system fits easily on any workbench. The Ion OneTouch 2 system integrates multiple manual template preparation steps into a single system. This enables parallel processing of multiple samples per day through modular design. It is flexible and supports scalable template preparation for all Ion semiconductor sequencing chips with up to 400 bases on the Ion 520/530 chips, up to 200 bases on the Ion 540 chip, up to 400 bases on the Ion PGM system and up to 200 bases on the Ion Proton system. The need for gas is eliminated by using a peristaltic pump. Chip compatibility: Ion 314 chip, Ion 316 chip, Ion 318 chip, Ion PI chip. High-throughput compatibility: Automated protocols, compatible for high-throughput, multiplexing. Library type: Fragment library, pair-mate library, target sequencing library, cDNA library. Multiplexing capability: Up to 384 barcode libraries. Runtime: Hands-on time: minutes, Ion PGM System: 5 hours total for template preparation