Product Description. This product offers a modular design and many configuration options. It allows for simplified sample preparation without washing and lysis options and prevents liquid clogging. Its unique applications enable complex protocols for a wide range of cell types and samples. It is a customized system with 4 lasers and 14 colors. The data transfer rate is up to 35,000 events/second and detects particles with diameters from 0.5 µm to 50 µm. The Attune NxT can be designed to accommodate the most common fluorochromes and fluorescent proteins used in flow cytometry to achieve the most panel matches. Multiple fluorescent proteins can be detected with an optional 561 nm laser. The Attune NxT flow cytometer is designed to use acoustically assisted hydrodynamic focusing to achieve sample throughput rates from 12.5 µL/min to 1 mL/min or 12.5–1,000 µL/min - up to 10 times faster than traditional hydrodynamic focusing systems and data acquisition speeds of 35,000 events/second. Acoustic focusing enables significantly higher sample rates, simplifying sample preparation, reducing protocol times and minimizing cell loss. This feature is useful for immunophenotyping protocols without washing or lysis using whole blood and for samples that are inherently low in concentration, such as CSF and stem cell preparations. As a personal flow cytometer, the Attune NxT AFC2 rivals the most complex multilasermulticolor instruments on the market today and meets the demands of the most complex experiments. Specifications. Laser. Violet: 405 nM (50 mW). Blue: 488 nM (50 mW). Yellow: 561 nM (50 mW). Red: 637 nM (100 mW). Filter. VL1: 440/50. VL2: 512/25. VL3: 603/48. VL4: 710/50RL1: 670/14. RL2: 720/30. RL3: 780/60YL1: 585/16. YL2: 620/15. YL3: 695/40. YL4: 780/60BL1: 530/30. BL2: 590/40. BL3: 695/40. Scattering. Forward scatter: 488/10 photodiode detector. Side scatter: PMT with 488/10. Control and analysis. Dell desktop with Intel CORE i7 with Windows 7. Attune NxT software version 2. 2nd Dell flat panel LCD monitor. Automatic maintenance. Power up (less than 15 min.), Power down, Rinse, Wash, Vent, Deep clean, Monthly decontamination. Gates. Standard and customizable gates. Graphic resolution. Publication quality images (customizable). Probe design. Sensible probe design minimizes clogging and carryover (less than 1%) and prevents damage to the instrument. Sample mixing. Sample mixing by pull-up to maintain sample versatility and cell viability. Laser. Bypass mode: Automatic shutdown extends laser life by 10xPre-aligned and welded fibers to laser interfacePre-aligned fiber to beam forming optics (BSO). Leverage flat-top lasers. • ~50% reduction in scattered light. • No problems with lateral shift in alignment