PCR

Appl.Biosystems 7900 HT

Special Price €10,710.00
-15%
Discount
until May 31, 2024
Regular Price €12,600.00
Excl. 19% VAT , excl. Shipping Cost
€12,744.90 incl. Vat
ID-number: 40149
Status: Stock unit?The device is in our stock, however, has to be inspected by our technician, in order to be able to guarantee complete function.
Shipping: 10-14 working daysiFor incoming orders until 12:00 (German local time). If customs handling is required, shipment will be delayed.
Description:
Applied Biosystems Fast Real Time PCR System 7900HT.
With PC and software SDS 7900HT V 2.3, for Win XP.
96-well block.
Accessories: various 96 well plates.
230 V. 50/60 Hz. 16 A.
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More Information
Objektnummer B00040149
ID-number 40149
Object name Appl.Biosystems 7900 HT
Status Lagergerät
Manufacture Appl.Biosystems
Shipping 10-14 working days

Status, terms of delivery and payment

Verification of devices

The second-hand devices are verified by Labexchange Service GmbH before delivery. You are receiving only fully functional devices.

Dispatch time

The stated dispatch times are the shortest possible ones for each article. The effective dispatch times can vary. The effective dispatch times will be stated in the order confirmation.

As a matter of principle, we are offering collective deliveries. The shipping time is calculated based on the position with the longest lead time. A partial delivery is possible on explicit request.

 

Shipping methods

Parcel services, forwarding agencies, self-pickup, delivery by Labexchange fleet.

 

Delivery information

Prices exclude shipping costs. Stated shipping costs are to be expected. Deviations are possible. If transport costs are not specified, please ask separately for them.

For deliveries to countries outside the EU, an additional fee of 70,00€ for export is charged. Note for international shipments: A proof of preference/EUR1 will not be issued by us. When self-collecting/ordering EXW from countries within or outside the European Union, 16% VAT will be retained as a deposit until we have received the corresponding confirmation of arrival/bill of delivery from the buyer.

 

Terms of payment

We do not accept payment by credit card, letter of credit, PayPal, etc. In each case the invoice amount is payable without deduction. Discount is not granted.

Country

Possible payment methods

Comment

DE, AT, CH

Payment by invoice, payment in advance

Payment by invoice is only possible for corporate clients.

NL, BE, LU

Payment by invoice, payment in advance

Payment by invoice is only possible for corporate clients

Other countries

Payment in advance

 

 

Our General Terms of Sale, Delivery and Payment are valid and are available for download here. 

The goods are offered subject to prior sale.

 

Definition of status

All articles are used articles, except an article is listed especially as a new device.

Status

Condition

Comment

Immediately available

Used The article is fully functional and in impeccable condition. It can be shipped immediately.
In stock

Used

The article is on stock. Our service technicians will verify the article before delivery. You are receiving only a fully functional article.

Published

Used

The article is still with the provider. After your order the article will be purchased and verified by us before being shipped to you. A certificate of operativeness as well as a service report are included in delivery.

New device

new

The article is brand new and unused. Regarding new equipment the guarantee/warranty conditions of the corresponding manufacturer apply.

Firma:             Appl.Biosystems

Modell:           7900 HT

 

 

The following illustrations and descriptions are referring to the instrument model and are drawn from brochures. They are not representing the delivery volume. The exact delivery content you will find only in the offering text.

 

 

Applied Biosystems 7900HT Fast Real-Time PCR System

 

•           Interchangeable blocks provide improved throughput and flexibility

•           Fast Real-Time PCR System deliversvers 96-well results in about 35 minutes

•           Custom Automation Accessory pro­vides 24-hour unattended operation

•           Powerful, flexible Standard and Enterprise Edition Software Packages provides tools for high­throughput data analysis

•           Enterprise Edition Software assists with 21 CFR Part 11 requirements

•           Hand-held and integrated bar code readers simplify sample tracking

•           Continuous wavelength detection from 500-660 nm allows the use of multiple fluorophores in a single reaction

 

lntroduction

The Applied Biosystems 7900HT Fast Real-Time PCR System is a high­throughput real-time PCR system that detects and quantitates nucleic acid sequences. This flexible system offers multiple block configurations, software choices, and an optional Automation Accessory, all designed to deliver the throughput and features you need. As your research expands, your system does, too. Key applications include gene expression quantitation and the detection of single nucleotide polymor­phisms (SNPs) using the fluorogenic 5" nuclease assay.

 

Fluorescence Detection

To induce fluorescence, the 7900HT System distributes light from an argon­ion laser excitation source to all sample wells. lt then directs the resulting (fluorescent emission through a spec­trograph to a charge-coupled device (CCD) camera. Emission wavelengths from 500-660 nm are monitored allowing the simultaneous detection of multiple fIuorophores.

 

System Components

7900HT Fast Real-Time PCR System

•           Peltier-based thermal cycling system with interchangeable block capability

•           Extended-life 488 nm argon-ion laser excitation source

•           Excitation light distributed to all wells via a dual-axis synchronous scanning head

•           Fluorescence detection via a spectro­graph and cooled CCD camera

•           Robot-compatible plate loading and unloading (Automation Accessory required to Ioad multiple plates without user intervention)

 

User-Interchangeable Blocks

The 7900HT system offers you a choice of blocks to meet your research needs:

•           lndustry standard 96-Well Block

•           lndustry standard 384-Well Block

•           TaqMan Low Density Array Block allows you to run TaqMan Low Density Arrays

•           Fast 96-Well Block delivers 96-well results in about 35 minutes

 

Automation Accessory

A Custom Zymark Twister Robot with five plate-stacking positions provides automatic plate-loading capability.

Each of the four input stacking posi­tions holds up to twenty-one 384-well plates or nine standard 96-well plates. The fifth position acts as an output stack.

Automation Accessory .performance plate handling errors minimizes at a frequency of less than 1% of total plates handled.

 

Sequence Detection Software

Both the Standard Edition and Enterprise Edition Sequence Detection System Software run on Windows XP operating system and are used for instrument control, data collection and analysis.

The upgrade to the Enterprise Edition Software includes access to two new analysis packages designed for high­throughput SNP Genotyping (SNP Manager) and Gene Expression (RQ Manager) as well as secure, robust and scalable data management in an Oracle database. This software also includes a full suite of features that comply with 21 CFR Part 11 guide­lines including data integrity, security auditing functions, electronic signatures, and electronic record-keeping.

 

Computer

Applied Biosystems supplies a Dell computer for use with the 7900HT system.

 

Installation Specifications

Using the TaqMan RNase P 384-Well or 96-Well Instrument \/erification Plate, the Applied Biosystems 7900HT Fast Real Time PCR System can dis­tinguish between 5,000 and 10,000 template copies with a 99.7% confidence level.

 

Assay Chemistry

Rapid assay development guide­lines are provided to ensure success when using the fluorogenic 5" nuclease assay and SYBR Green 1 dye double-stranded DNA binding assay.

 

Default primer and probe concen­trations are valid for multicolor SNP assays using TaqMan MGB probes, and single color quantitation assays using TaqMan probes or the SYBR Green 1 double-standard DNA binding dye assay. Assay optimization is recommended for multiplex quantitation assays to minimize PCR competition.

 

Reagents and Disposables

A complete line of reagents and consumables is available for use with the Applied Biosystems 7900 HT Real-Time PCR System.

 

TaqMan Genomic Assays

Applied Biosystems provides pre­formulated, ready-to-use, quality-tested, 5" nuclease assays for use with the 7900 HT system (see table below).The TaqMan Low Density Array is also available for use on the 7900HT sys­tem and is used to analyze tens to hundreds of targets with multiple sam­ples. lt contains user-selected, invento­ried TaqMan Gene Expression Arrays loaded and dried-down into a 384 micro-well format.

 

 

TaqMan Genomic Assays

Assay Details

Application

Assay Formats

Gene Expression

SNP Genotyping

TaqMan Genomic

Assays

Yes

Yes

Custom TaqMan

Genomic Assays

Yes

Yes

Genome Availability

Number of Assays

Human

> 204,000

> 1,990,000

Mouse

> 179,000

N/A

Rat

> 128,000

N/A

URL (assays can be

ordered online only)

www.allgenes.com

Vvwvv.allsnps.com

 

 

Demonstrated Performance

Specifications

Fast 96-Well Block

Standard 96-Well Block

Dynamic range

9 logs of linear dynamic range using the human 18S rRNA TaqMan Gene Expression Assay

9 logs of linear dynamic range using the human 18SrRNA TagMan" Gene Expression Assay

Sensitivity

Detection of 10 starting copies of the RNase P gene from human genomic DNA in a 20 µL reaction volume

Detection of 10 starting copies of the RNaseP gene from human genomic DNA in a 25 µL reaction volume

Time to perform 40 cycles using the TaqMan RNase P

33 minutes to perform 40 cycles using the TaqMan RNase P Fast instrument verificationn plate

110 minutes to perform 40 cycles using the TaqMan Rnase P instrument verification plate

 

7900 HT System Throughput

System

Real-Time Analysis

End-point Analysis

 

7900HT System with 384-well block and Automation Accessory using Standard Thermal Cycling Prototal

> 5,000 sample wells/day

> 10,000 sample wells/hour

 

Block Applications

Block Type

Absolute

Quantitation

Relative

Quantitation

Allelic

Discrimination

Standard 96-well

Yes

Yes

Yes

Fast 96-well

Yes

Yes

No

384-well

Yes

Yes

Yes

TaqMan Low Density Array

Yes

Yes

No

 

Instrument Dimensions

Dimension

7900HT Fast Real-Time PCR

System with Automation

Accessory

7900HT Fast Real-Mine PCR System without Automation Accessory

Width

125 cm (49 in)

(with drawer in open

position) 72 cm (28 in)

Depth

84 cm (33 in)

84 cm (33 in)

Weight

64 cm (25 in)

64 cm (25 in)

Weight

114 kg (250 Ibs)

82 kg (180 Ibs)

 

 

Product group: PCR

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