manufacturer:       Hewlett Packard

model:                   1046A

annotation:           Dokumente engl.

Introduction

The most popular liquid chromatographic detectors are those which operate on physical principles, such as absorption or emission of light or refractive Index changes. Of these, luminescence detectors are the most sensitive and selec­tive, with fluorescence detectors particularly popular. The techniqu, can be applied to detect the native fluorescence of many organic molecules as well as the emission from fluorescently-labeled deriva­tives of non-fluorescing -fluorescin compounds. Indeed with improved autosampling devices, chemical derivatization with fluorescent reagents can be perfornied online immediately before the column. For the best results, the detector should be equipped with gratings before and after the flow cell for programming of excitation and emission wavelengths. This, among other things, is one of the features that makes the Hem-lett­Packard HP 1046A fluorescence detector the ideal choice for highly sensitive work. The detec. tor can be added to an existing liquid chromatograph as a stand­alone unit or integrated in an automated HPLC System, with integrator control or PC-based method programming on the Hewlett-Packard HPLC ChemStation.

Feature focus

Grating—grating opfts            Excitation and emission wavelength settings in 1-nm steps from 190 to 800 nm

Pulsed xenon lamp                Immediale readiness upon starting, even for measurements at highest sensitivity

Scanning of λ _ex .and λ _em         Excitation and emission wavelength method development.

Run-time programming of     Optimum sensitivity for different analytes in a single run

λ _ex .and λ _em

Optimized cell design            Illuminates practically all of the cell chamber for highest sensitivity. Cell volume of 5 pl suitable for narrow-bore HPLC

Phosphorescence and          Versatility in application

chemiluminescence modes

Built-in diagnostics and          Leak drainage, shut-down Signal to pump and reset

safety features                       function

Test cell                                 For verifying and calibrating wavelength accuracy in GLP regulatory work.

HP3396 Series ll integrator    Parameter storage on the integrator as method fites.

Control                                   Printing of method fites with the resulting chromatograms for regulatory requirements.

HPLC ChemStation control   Control and storage of all chromatographic settings, multi­method sequencing, printout of customized reports. Creation of spectral libraries containing excitation and emission scann with HPLCII ChemStation

Specifications

Excitation wavelengths

Time-programmable λ

190 - 800 nm and zero order, standard 12-nm bandwidth, optional 25- and 50 nm.

1200 lines/mm, blazed at 225 nm.

Emission wavelengths

Time-programmable λ

190 - 800 nm and zero order, standard 25-nm bandwidth, optional 12-, 50- and 140 nm. 1200 lines/mm, blazed at 450 nm.

Wavelength setting resolution

  1 nm

Wavelength repeatability

±1 nm

Wavelength accuracy

± 2 nm

Noise

Using λ _ex 250 nm (12-nm band­width), λ _em 400 nm (25-nm band- width), 370-nm cut-off filter, 1-s risetime and 220-Hz pulse fre­quency, noise is equivalent to 40 fg of anthracene in 5 µl of methanol.

Light source

Xenon flach lamp

Pulse frequency

220 Hz (5 W), 110 Hz (2.5 W) or 55 Hz (1.25 W)

Monochromator scan Speed

Selectable 50-, 25-,12-,6-,3- or 1.5 nm/s

Photomultiplier tube

Standard S5 response (200 - 650 nm)

Photomultiplier tube Input voltage

Time-programmable, selectable 250 - 1100 V

Flow cell

5-µl volume, maximum pressure 80 bar

pH range

2.3-9.-5

Communications

Outputs: ready signal. In- and outputs: starr, stop and shut down Signals. Optional Interface for HP-IB and RS-232C.

User interface

Vaeuum fluorescent display, 32 characters, brightness automati-

cally adjusted according to room lighting

Analog output

0 - 1000 mV, source impedance

1 kW, 0 - 100 mV, source impedance 100 W

Rise time (10 % - 90 %)

0.1 - 4 seconds

Environment

0 - 55 °C, 5 - 95 % relative humidity (40 °C)

Power

100 - 120 V _AC ~c, 220 – 240V _AC

live frequency 48 - 66 Hz, power

consumption 50 V maximum.

Dimensions

430 mm (17 ") x 370 mm (14.5 ") x

150 mm (21.3 ") width x depth x height.

Weight

13 kc (29 lbs)