Information provided

Kinetic and affinity data (KD, ka, ka, R50), binding level ranking, association and dissociation level ranking, binding level vs. stability (binding strength), specificity

Data presentation

Result tables, result plots and real time monitoring of interactions

Throughput

Typically 200 samples/24 hours (Hit Selection)

Automation

24 hour unattended operation

Sample type

Low or high molecular weight compounds, e.g. in DMSO containing buttere.

Molecular weight detection

>100 Da (ligand and analytes dependent)

Sample concentration

Sub nM to mM sample concentrations

Required sample volume per analysis

30 - 370 µl (application dependent)

Vial dead volume

10 µl (for microplates supplied by Biacore)

Target protein consumption

Typically 5-20 µg per immobilization

Compound consumption

Typically 1 - 5 µg per determination

Assay flow rate

10, 30 or 90 µl/min

Sample/reagent capacity

One 384 well or one 96 well microplate + 33 reagent vials + 2 reagent bottles

Analysis temperature

4 - 45 °C (maximum 20 °C below ambient) 

Sample temperature          

From ambient temperature to 35 °C

Sample refractive index range

1.33.- 1.40

in-Iine reference subtraction

Automatic

Typical working ranges, kinetics:

Association rate constant (ka)           Up to 5 x 106 M-1s-1 (and higher for macromolecular analytes)     

Dissociation rate constant (kd)          10-4-1 s-1 (dissociation time limited to 1000 s)Affinity (KD from kd/ka)                       

Typically 100 µM - 10 pM (analyte and target dependent)

Flow cell configuration

Two flow cells, each with two independent detection spots and an in-line reference

Baseline noise

Typically <0.1 RU (RMS)

Baseline drift

Typically <0.3 RU/min in ambient temperature range 15.35 °C

Dimensions (footprint, excluding System Controller)

600 x 620 x 690 mm (W x D x H)

weight

62 kg (137 Ibs)

Power 

100 - 240 V AC, 50.60 Hz,