Becton Dickinson FACSCalibur
| Objektnummer | B00014408 |
|---|---|
| ID-number | 014408 |
| Object name | Becton Dickinson FACSCalibur |
| Status | Stock unit |
Product group: Flow cytometer
Status, terms of delivery and payment
Verification of devices
The second-hand devices are verified by Labexchange Service GmbH before delivery. You are receiving only fully functional devices.
Dispatch time
The stated dispatch times are the shortest possible ones for each article. The effective dispatch times can vary. The effective dispatch times will be stated in the order confirmation.
As a matter of principle, we are offering collective deliveries. The shipping time is calculated based on the position with the longest lead time. A partial delivery is possible on explicit request.
Shipping methods
Parcel services, forwarding agencies, self-pickup, delivery by Labexchange fleet.
Delivery information
Prices exclude shipping costs. Stated shipping costs are to be expected. Deviations are possible. If transport costs are not specified, please ask separately for them.
The stated transport and packing charges apply to the most favorable route if transport and are to be understood as subject to verification due to unexpected cost increases. By reason of unpredictable events, cargo rates and delivery times can change at any time and therefore have to be adapted to the recent situation. Import formalities and possible customs charges will be borne by the purchaser. Incoterm coding according to Incoterms 2010: For persons who collect the devices themselves: EXW, for dipatch by sea: CFR, by air freight: CPT, other shipments: DAP. Note for international shipments: A proof of preference/EUR1 will not be issued by us. When self-collecting/ordering EXW from countries within or outside the European Union, 16% VAT will be retained as a deposit until we have received the corresponding confirmation of arrival/bill of delivery from the buyer.
Terms of payment
We do not accept payment by letter of credit, PayPal, etc. In each case the invoice amount is payable without deduction. Discount is not granted.
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Country |
Possible payment methods |
Comment |
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DE, AT, CH |
Payment by invoice, payment in advance, paymet by credit card |
Payment by invoice is only possible for corporate clients. |
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NL, BE, LU |
Payment by invoice, payment in advance, payment by credit card |
Payment by invoice is only possible for corporate clients |
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Other countries |
Payment in advance, payment by credit card |
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Our General Terms of Sale, Delivery and Payment are valid and are available for download here.
The goods are offered subject to prior sale.
Definition of status
All articles are used articles, except an article is listed especially as a new device.
|
Status |
Condition |
Comment |
|
Immediately available |
Used | The article is fully functional and in impeccable condition. It can be shipped immediately. |
| In stock |
Used |
The article is on stock. Our service technicians will verify the article before delivery. You are receiving only a fully functional article. |
|
Published |
Used |
The article is still with the provider. After your order the article will be purchased and verified by us before being shipped to you. A certificate of operativeness as well as a service report are included in delivery. |
|
New device |
new |
The article is brand new and unused. Regarding new equipment the guarantee/warranty conditions of the corresponding manufacturer apply. |
|
Labprocure |
Used |
Labprocure GmbH, as the advertiser, is responsible for the content of this device offer. Labprocure assumes liability for the offers advertised here and for the photos and offer texts included. Labprocure GmbH, Bruckstraße 58, 72393 Burladingen. |
manufacturer: Becton Dickinson
model: FACSCalibur
The following illustrations and descriptions refer to the instrument model and are drawn from brochures. They do not represent the scope of delivery. Please refer to the text of the offer for the exact scope of delivery.
Optics
Excitation Optics
Optical platform
Fixed optical assembly
Lasers
Air-cooled argon-ion laser, 15 milliwatt, 488 nm; life expectancy >5,000 hours.
Optional second laser: nominally 635 nm.
Beam geometry
Prismatic expander and achromatic
spherical lens providing 22 x 66-μm
elliptical beam for argon-ion laser.
Nominally 15 x 61-μm elliptical beam
for red-diode laser.
Emission Optics
Optical coupling
Quartz cuvette coupled to emission lens by refractive index matching optical gel for optimum collection efficiency.
Background rejection
Obscuration blade and slit for minimizing unwanted laser radiation at the detector.
Forward scatter detector and filter
High-performance solid state silicon detector with 488-nm bandpass filter for clear signal detection and red-diode (635-nm) laser signal rejection.
Side scatter detector
High-performance photomultiplier using Brewster-angle beam splitter in the emission optical train.
Fluorescence detectors and filters
Up to four high-performance, high dynamic range photomultipliers with bandpass filters: 530 nm (FITC), 585 nm (PE/PI), and >670 nm (PerCP) with base unit, and optional 661 nm (APC) with FL4 option.
Performance
Fluorescence
Fluorescence Sensitivity
Estimated detection limit: 750 molecules of equivalent soluble fluorescein.
Fluorescence resolution
Coefficient of variation in FL2-Area of <3%, full peak for propidium iodidestained chicken erythrocyte nuclei.
Forward and Side Scatter
Forward and side scatter sensitivity
Separation of fixed platelets from noise.
Forward and side scatter resolution
Optimized scatter performance for resolving lymphocytes, monocytes, and granulocytes.
Signal Processing
Workstation resolution
1,024 channels on all parameters.
Dynamic range
Logarithmic amplifiers for SSC, FL1, FL2, FL3, and FL4 (with FL4 option), providing four log decade range.
Fluorescence compensation networks
Compensation for fluorescence spectral overlap between FL1 and FL2, between FL2 and FL3, and between FL3 and FL4
channels (with FL4 option).
Pulse processing
Width and area measurements for discriminating doublets; available for all fluorescence parameters.
Time
Time available correlated to any parameter for kinetic experiments or other applications.
Fluidics
General operation
Front key panel control in three modes:
RUN, STNDBY, and PRIME; automatic standby mode for conserving sheath fluid by stopping sheath flow when no sample tube is installed.
Fluid reservoirs
Easily accessible 4-L capacity sheath and waste containers housed in a convenient pull-out drawer; level detectors for automatically indicating low levels of sheath or high levels of waste.
Sample flow rates
Three selectable flow rates of 60 μL/min, 35 μL/min, and 12 μL/min; regulated and monitored pressure difference between sheath and sample; particle velocity in flow cell: approximately 6 meters/second.
Quartz cuvette
Internal cross-section: rectangular 430 x 180 μm; antireflection coated external surfaces for maximum transmission of laser light.
Sample concentration
Single-cell suspension of 105 to 2 x 107 particles/mL recommended range.
Data Management
Workstation
BD FACStation™ Mac Pro computer
Processor
2.8-GHz Quad-Core Intel® Xeon® processor
Memory
2 GB RAM
Level 2 cache
4 MB per processor
Data storage
320-GB 7200-rpm Serial ATA, 3Gb/s
Networking
On-board Ethernet, built-in AppleTalk® Networking, and Apple® File Sharing
Optical drive
16x SuperDrive
Monitor
17-inch LCD monitor (optional)
Data file structure
Flow Cytometry Standard (FCS) 2.0/3.0
ASCII results file for data export
Sample Loading
Sample delivery
Tube-lifter design with multiple sensors that verify rack identification and tube position with optional BD FACS™ Loader.
Rack capacity
40 (12 x 75-mm) tubes per rack.
Rack support
Up to 16 racks per BD FACS Loader.
Data entry
Sample information, reagent panels, and rack information can be defined for up to 640 tubes (40 tubes x 16 racks) at a time.
Loader manager
Automated control through BD™ Worklist Manager software.
Barcode scanner (optional)
Automates data entry for Codabar, Code 39, Interleaved 2 of 5, Code 2 of 5, and Code 128.
Mixing mode
Adjustable high-energy and low-energy mix.
Installation requirements
Dimensions (W x D x H)
Sensor module
91.4 x 61.5 x 67.3 cm
(36 x 24.2 x 26.5 in)
Height with cover option
124.5 cm (49 in)
Computer
48 x 41 x 58 cm (19 x 16 x 23 in)
Printer
48 x 41 x 58 cm (19 x 16 x 23 in)
Weight
Sensor module
109.1 kg (~240 lb)
Computer
varies with configuration
Power
US: 120 VAC ±10%, 50/60 Hz ±2 Hz,
Current: 20 amp maximum outside
US. External transformer needed for
100 VAC ±10%, 50/60 Hz ±2 Hz, and
220/240 VAC ±10%, 50/60 Hz ±2 Hz.
Water supply
None required
Air supply
None required
Heat dissipation
BD FACSCalibur system with all options
6,500 BTU/h
Temperature
16–29°C (60–85°F)
Humidity
10% to 90% relative non-condensing
Air filtering
No excessive dust and smoke
Lighting
Optics and detectors shielded from room
lighting
