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Appl.Biosystems Voyager-DE STR

ID-number: 009171
Status: Stock unit
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Applied Biosystems Voyager-DE STR Biospectrometry Workstation. MALDI-TOFMass Spectrometer with Reflector Analyzer. YoC.: 2000.Newprice: 296.550,- Euro
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Objektnummer B00009171
ID-number 009171
Object name Appl.Biosystems Voyager-DE STR
Status Stock unit

Product group: Mass spectrometers

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Firma:              Appl.Biosystems

Modell:            Voyager-DE STR

Kommentar:   Dokumente engl.

The Voyager-DE STR Biospectrometry Workstation (Figure 1-3) is a floor-standing MALDI-TOF (matrix-assisted laser desorption ionization time-of-flight) mass spectrometer that includes a reflector analyzer.

Figure 1-3 Voyager-DE STR Biospectrometry Workstation

Biospectrometry

Biospectrometry is the application of mass spectrometry in the field of the life sciences.

This field uses fast chromatographic techniques, enzymatic chemistries, and surface chemistries and combines them with mass spectrometry and advanced software to better enable biomolecular research and facilitate data interpretation.

Features

Features of the Voyager-DE STR Biospectrometry Workstation include:

•     High-speed digitizer options and laser rates for optimum sample throughput

•     Positive and negative ion detection

•     Modular horizontal design

•     High-performance reflector

•     Post-source decay (PSD) analysis capability

•     Linear or reflector mode operation

•     Ion path length:

•     Linear mode-2.0 meter

•     Reflector mode-3.0 meter

•     Timed ion selection

•     m/z range in excess of 300 kDa

•     Sensitivity to less than 5 femtomoles with dried droplet application

•     Variable two-stage ion source

•     Automated single-plate sample-loading system; sample plates of various formats available

•     Ion focusing lens technology for increased sensitivity (serial number 4154 and later)

•     Video camera and monitor for sample viewing

•     Low mass gate matrix suppression

•     Intuitive Microsoft® Windows NT-based software

•     Collision-induced dissociation (CID) option

•     Second laser option

This section describes:

• System components

• Mass spectrometer

• Vacuum System

• Front panel indicators

• Computer components

Parts of the Voyager-DE STR system include:

•     Mass spectrometer

A time-of-flight mass spectrometer, described in Section 1.6.2, Mass Spectrometer. The high-vacuum system of the mass spectrometer is described in Section 1.6.3, Vacuum System.

•     Computer/Data System

A computer that operates the Voyager control software and the Voyager processing software. You control the mass spectrometer using the computer.

•     Digitizer (not shown)

An analog-to-digital converter that allows the signal from the mass spectrometer to be transferred to the computer.

The standard digitizer provided is a 4 GHz digitizer.

•     Video monitor

A monitor that displays a real-time video image of the sample spot for examination during acquisition.

•     Control stick

A peripheral device that controls position of the sample plate in the mass spectrometer. Allows you to start and stop acquisition and transfer data to the processing software.

The parts of the Voyager-DE STR Biospectrometry mass spectrometer are shown in Figure 1-18.

Figure 1-18 Voyager-DE STR Mass Spectrometer

Table A-7 Voyager-DE STR Performance Specifications for Installation

Condition

Specification

Resolution (FWHM-Full Width at Half Maximum) in Delayed Extraction mode

Linear mode:

• ≥ 3,000 for Angiotensin I (1.3 pmol/µl)

• ≥ 3,500 for ACTH (18-39) (1.5 pmol/µl)

Reflector mode:

• ≥20,000 for bovine insulin (3.5 pmol/µl)

• ≥1,200 for E. coli Thioredoxin (2.75 pmol/µl)

• ≥12,000 for Angiotensin (2.0 pmol/µl)

• ≥12,000 for ACTH Clips

• ACTH 1-17 (2.0 pmol/µl)

• ACTH 18-39 (1.5 pmoi/µl)

• ACTH 7-38 (3.0 pmol/µl)

[M+H] + ion analyzed.

Signal-to-Noise Ratio

• ≥ 200:1 for IgG (0.6 pmol/µl)

• ≥ 100:1 for BSA (4 pmol/µl)

Sensitivity

Routine detection of 5 fmol of neurotensin with a signal-to-noise ratio >80:1

Table A-7 Voyager-DE STR Performance Specifications for Installation

Condition

Specification

Mass Accuracy in Delayed Extraction mode

(difference between the mean of six measurements and the theoretical mass of the sample)

Linear mode:

• External Calibration - 0.05%

• Internal Calibration - 0.02%

Reflector mode:

• External Calibration - 0.008%

• Internal Calibration - 0.001%

Mass Accuracy of Fragment Ions

+/- 1 Da

Resolution of PSD Precursor (Timed) Ion Selection

80

Table A-8 Voyager-DE STR Mass Spectrometer Specifications

Condition

Specification

Mass range

≥300,000 Da

Upper limit set by suitably ionized biomolecule.

Reflector

Single-stage with optimized optics for PSD Analysis

Flight tube (horizontal)

• Linear mode - 2.0 m

• Reflector mode - 3.0 m

Ion source

Two-stage

Ion source voltages

Tunable:

• Accelerating Voltage - Up to 25,000 V

• Grid Voltage - Range determined by Accelerating Voltage

Laser

Nitrogen, 337 nm, 3 ns pulse, 20 Hz maximum firing rate

Actual laser firing rate dependant on digitizer

Digitizer

2 GHz digitization for enhanced resolution

Table A-8 Voyager-DE STR Mass Spectrometer Specifications (Continued)

Condition

Specification

Vacuum system

• Dual differential turbomolecular pumping for ultrahigh vacuum

• Multi-vacuum gauge capability for independent monitoring of source and analyzer regions

Ion detection

Positive and negative

Sample analysis

• Automated single-plate sample-loading system; sample plates of various formats currently available

• Manual control using control stick or mouse

• Sequence Control software for automated analysis

Dimensions

• 34 inches (87 cm) deep

• 94 inches (239 cm) wide

• 46 inches (117 cm) high

Table A-9 Voyager-DE STR Miscellaneous Specifications

Condition

Specification

Environmental

• Operating temperature-10-25°C

• Relative humidity-10-80%, non-condensing

• Altitude-≤ 2,000 m (6,500 ft) above sea level.

• Installation category (overvoltage category)-II, classified as portable equipment.

• For indoor use only.

• Pollution degree rating-2, and may be installed in an environment that has nonconductive pollutants only.

Computer

Minimum configuration:

• Pentium III

  500 MHz, with 9 GB hard disk and 128 MB

  RAM (random access memory)

• 17-inch color monitor

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