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Appl.Biosystems BioCAD 700E

ID-number: 010123
Status: Stock unit
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Description:
Applied Biosystems Chromatography-System BioCAD 700E. Semipräp. Flowcell.Fraction collector Advantec SF 2120. YoC: 1999.
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Objektnummer B00010123
ID-number 010123
Object name Appl.Biosystems BioCAD 700E
Status Stock unit

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Definition of status

All articles are used articles, except an article is listed especially as a new device.

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Condition

Comment

Immediately available

Used The article is fully functional and in impeccable condition. It can be shipped immediately.
In stock

Used

The article is on stock. Our service technicians will verify the article before delivery. You are receiving only a fully functional article.

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Used

The article is still with the provider. After your order the article will be purchased and verified by us before being shipped to you. A certificate of operativeness as well as a service report are included in delivery.

New device

new

The article is brand new and unused. Regarding new equipment the guarantee/warranty conditions of the corresponding manufacturer apply.

Labprocure

Used

Labprocure GmbH, as the advertiser, is responsible for the content of this device offer. Labprocure assumes liability for the offers advertised here and for the photos and offer texts included. Labprocure GmbH, Bruckstraße 58, 72393 Burladingen.

Firma:              Appl.Biosystems

Modell:            BioCAD 700E

Kommentar:   Dokumente engl.

Overview

PerSeptive Biosystems BioCAD ® 700E Workstation for Perfusion Chromatography ® is a computer-aided chromatography workstation that integrates all aspects of biomolecule purification with Perfusion Chromatography technology.

The BioCAD 700E Workstation is designed for:

•     Chromatographic method execution —Routine execution of chromatographic methods for the purification and analysis of biomolecules.

•     Systematic method development —The goal of method development is to optimize conditions for your application. Systematic method development makes it easy to identify variables that affect your application, systematically change the variables, observe the effects, and use the information to optimize chromatographic conditions.

The BioCAD 700E Workstation provides the following features:

•     Simplicity —Microsoft ® Windows ® -based software that is intuitive and easy to use. You can efficiently develop and execute separations for analytical and preparative applications.

•     Full Process Capability —Full capability from sample injection through fraction collection, including automated column washing and regeneration.

•     Speed —Chromatographic separations in as little as one to three minutes, when using POROS ® media and columns.

•     Versatility —Multiple column configurations and injection mode options.

•     Gradient Capability —Six-buffer/solvent capability, with simultaneous blending between any four buffers/solvents.

•     Detection —Monitoring of pH, conductivity, dual-wavelength UVNis absorbance, fluorescence (optional), and system pressure.

•     Templates —Predefined templates for systematic method development of key variables such as pH, gradient slope, and sample load.

•     Data Analysis —Data analysis of single data files or groups of data fites.

•     SCOUT ® Column Selector —Automated multi-column switching device that allows flow to be diverted to any one of six attached columns (optional).

Parts of the system

The BioCAD 700E Workstation includes:

•     Front panel indicators and controls

•     Buffer/solvent and pump system

•     Sample Ioader

•     Columns and plumbing

•     Detector and monitors

•     Fraction collection

•     Computer components

Figure 1-2 BioCAD 700E Workstation

Specifications

Condition

Specification

Weight

275 pounds (125 kg)

Space requirements

Space required for workstation, computer monitor, keyboard,

mouse, and buffers:

•     Height—36 inches (91 cm)

•     Depth—36 inches (91 cm)

•     Width—72 inches (183 cm)

Pumping system

Dual piston, positive dispiacement with 6 inlets, pressure

safety, and built-in seal flush

Flow rate

0.2–60 mI/min

Accuracy is +/- 1% of setpoint or 0.05 mI/min (whichever is

greater)

Maximum system pressure

3,000 psi (204 bar, 20.4 MPa)

Gradient capability

Up to six solvents, with Iow-pressure quaternary blending in

Percentage, Eluent, or pH blend modes with 0.1% increments.

Compositional accuracy: ± 1%

Sample handling

Six injection modes:

•     Autoloader—Up to four sample channels, with a sample

     loading range of 1 µl to 25 ml, ± 1% accuracy at full stroke

•     Direct Autoloader—Reduces prime volume (minimum 5

     µl), single sample channel, with a sample loading range of 1 µl to 25 ml, ± 1% accuracy at full stroke

•     Manual—Eliminates prime volume, needle port adapter

     included

•     Pump—Unlimited volume loading

•     Integrated Autosampler (optional)—Inject from multiple

     samples, with complete control over autosampler

     functions

•     Direct Wire Autosampler (optional)—Inject from

     multiple samples, control autosampler from autosampler

     front panel

Column sizes

•     POROS ® Perfusion Chromatography ® columns—2.1      to 25 mm diameter

•     Conventional chromatography columns—no limit on

     column size


Column configurations

•     Single column, with bypass and reverse

•     Tandem columns, with bypass

•     Three columns

•     Low dispersion

•     Direct control

NOTE: Use up to eight columns with optional SCOUT Column Selector.

Fraction collection

•     Internal fraction/waste valve for manual and automated

     control of single fraction collection

•     Integrated control of optional external fraction collectors,

     which accommodate tubes or larger bottles. Tube-to-tube switching time is 0.1 seconds.

Materials

Biocompatible and solvent-resistant—PEEK, Teflon ® , Kel F ® , Kynar ® , sapphire, stainless steel, aluminum oxide, ruby

Auxiliary inputs and outputs

•    Inputs—2 TTL

•    Outputs—2 TTL

Auxiliary driver outputs

•    Spareout LC—12 V (0.25 A)

•    Spareout HC—24 V (1 A)

Detection

Condition

Specification

Pressure

Transducer on pump discharge—0 to 3,000 psi

Auxiliary input

12-bit A/D for additional detector

Monitor bypass

Automated valve switches pH/conductivity probes in and out of line

pH probe

•    Range—2 to 12 pH units

•    Flow cell volume—50 µl

Conductivity

probe

•    Range—0 to 200 mS

•    Flow cell volume—7 µl

UV/Visible

Model 201 (single wavelength)—190 nm to 800 nm

Model 205 (dual wavelength)

•    190 nm to 800 nm (single wavelength range)

•    190 to 450 nm OR 366 to 700 nm (dual wavelength ranges)

Flow cells available:

•    Analytical—6 mm path length, 9 µl volume, 45 mI/min flow

    rate maximum

•    Semi-prep—3 mm path length, 4.5 µl volume, 100 mI/min

    flow rate maximum


Miscellaneous

Standard

Specification

Hardware

(standard)

•         Internal Pentium/133 MHz processor (minimum)

•         3.2 gigabyte hard disk drive (minimum)

•         64 megabyte RAM (minimum)

•         3.5-inch floppy disk drive

•         14-inch Super VGA color monitor

•         Keyboard

•         Mouse

•         Network card

•         SCSI card

Hardware

(options)

•         17-inch color monitor

•         21-inch color monitor

•         Laser and color printers

•         SCOUT Column Selector

•         External detector (UV/Vis or fluorescence)

•         Fraction collector

•         Autosampler

•         Kits of selected POROS columns

Software

(standard)

•         BioCAD Software

•         Windows 95 Version OSR 2.5

Software

(options)

•         SupervisorTM Software (standalone software version)

•         Microsoft Office 97

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