The combination of mass spectrometry (MS) and nanoflow liquid chromatography (nanoLC) is an essential part of proteomics research. The strength of nanoLC-MS is that it enables the analysis of small amounts of biological samples with high sensitivity. The diversity of chromatographic phases and column arrangements has led to the widespread adoption of nanoLC-MS to address a wide range of biological problems. However, the low solvent flow rates in nanoLC make chromatographic optimization more challenging than in higher flow rate applications. Therefore, it is important that the Nanoflow interface to the mass spectrometer is robust, user-friendly and flexible to ensure optimal performance in a wide range of applications. There are many examples of established and emerging applications that place different requirements on the nanoLC source and interface. The broad distribution of hydrophobic and hydrophilic peptides in protein digests typically requires the use of a broad LC solvent gradient to improve protein sequence coverage. Some applications require the use of nanoLC tips with taper emitter tips in which the chromatographic material is directly packaged. Therefore, it is essential to have a versatile and stable nanoflow ESI system that is robust to different solvent compositions, chromatographic formats and media, and even polarizations when analyzing proteomic samples in automated mode. Key Features of NanoSpray III Source:. - Simplified emitter tip and column replacement with connections that can be tightened with your fingers and quick release of the spray assembly. - Rail mounted sliding connection allows the use of any length emitter tips. - Fog gas and HV connect directly to the source. - Spray assembly can be removed without disconnecting from gas or HV. - Improved lighting and cameras for continuous spray visualization (new compact LCD monitors). - Fixed spray angle (~25°) makes it easier to adjust the spray and reduces contamination of the interface. - Source and nanoDCI heated interface compatible with SCIEX QTRAP and TripleTOF systems. - Optiflow interface with Nanoflow heater and cover plate compatible with the TripleTOF 6600 system. - Compatible with all nanoLC systems and column configurations, including sorbent-packed taper-emitter tips for high-quality separations Labprocure is responsible for this device.